Device
FlipFlop

Part:BBa_K907002:Design

Designed by: Dong-hui Choe, Soo-in Lee   Group: iGEM12_KAIST_Korea   (2012-09-20)


Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 75
    Illegal NheI site found at 98
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 42
    Illegal BsaI.rc site found at 125


Design Notes

No


Source

Promoter and RBS are derived from BBa_J23119 and BBa_B0034, respectively. Whole construct was de novo synthesized by Bioneer.

References